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Revision and evaluation of sites with current and historical occurrence of selected species \kur{Orchidaceae} in Bohemian Paradise (Czech: Český ráj)
ALBRECHTOVÁ, Linda
The subject of this diploma thesis was the research of vegetation at localities in the Protected Landscape Area Bohemian Paradise. There were choosen areas: Natural Monument Podloučky and Protected Area Fialník, where grows an orchid Gymnadenia conopsea subsp. montana. There were made 60 phytocenological samples, and described: biotop, altitude, slope, huminidy and exposure. There were found four orchid species in the localities. Phytocenological samples were compared with each other. It has been shown that differences between localities are greater than the differences between orchid and non-orchid samples. Significant differences were also found in comparing the coverage in the floor between locations and abundace of species in each floor.
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Utilization of trehalose in orchids: evolution of trehalase genes
Šoch, Jan ; Ponert, Jan (advisor) ; Hála, Michal (referee)
All orchid species studied so far have been shown to participate in orchideoid mycorrhizal symbiosis. Morover, this symbiosis is absolutely vital component of their life cycle. Exchange of nutrients occurs between symbionts where the fungi provides the orchid with energy and carbon supply at least in its early developmental stages. This study focuses on the possible role of trehalose in this transfer. In vitro experiments have showed in five species from three different subfamilies of Orchidaceae family that they can utilize trehalose comparably with sucrose and glucose. Thus, the ability of trehalose utilization seems to be conserved among orchids. Trehalase enzyme activity was localized histochemically in orchid mycorrhizas. The activity strongly colocalized with colonized tissue supporting a hypothesis that trehalose transfer occurs in this site and is mediated by trehalase. Using bioinformatic methods, trehalase gene duplications were identified in many taxons of Embryophyta including three orchid species. Interestingly, highest number of trehalase gene copies was identified in genome of orchid Dactylorhiza majalis. Trehalose utilization, high trehalase activity in mycorrhizas and trehalase gene duplications in some orchids together indicate that trehalose transfer in orchid myccorhizas...
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Rozdíly v účinnosti a fytotoxicitě vybraných insekticidů pro ošetření orchidejí
Burianová, Ilona
The Bachelor thesis "The differences in the efficiency and the phytotoxicity of selected insecticides for treating orchids" deals with the orchid pests and various kind of protecting measures against them. Efficiency of the individual preparation and plant reactions to treatment are verify too. Pests cause important deterioration of quality. Knowledge of pests and pathogens biology, their harmfulness economic importance is prerequisite for effective protection against them.
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